BACTERICIDAL ACTIVITY OF THE NEW DISINFECTANT "DIOLAYD" UNDER ITS EFFECT ON DIFFERENT TYPES OF BACTERIA

Abstract

Practice demonstrates that over the last decade, one of the main methods of combating, preventing from zoonotic infectious agents and maintaining of the epizootic well-being in the livestock One Health Journal. 2023. Том 1. Номер 1 14РОЗДІЛ 1 industry of Ukraine, and in the poultry industry in particular, remains disinfection with the constant desire of manufacturers to develop cheap, multi-component, bactericidally effective disinfectants. Experimental "in vitro" studies of the developed disinfectant "Diolide" at the first stage was tested for bactericidal activity and lack of bacteriostatic effect. The suspension method was used on the models of test cultures of microorganisms: gram-positive - Staphylococcus aureus ATCC 25923 and gram-negative - Pseudomonas aeruginosa ATCC 15442, which corresponded to all the main typical properties regarding their species identification and showed resistance to the action of standard disinfectant solutions - 0.02% pH chloramine after exposure for 5 minutes; 2.0 % aqueous hydrogen peroxide and 0.03 % aqueous glutaraldehyde for exposure for 10 minutes; 0.025 % of ADBAH district for exposure of 20 minutes. Daily bacterial suspensions of test cultures of the specified microorganisms were used at the concentration of 0.5 OD according to the optical standard of turbidity according to McFarland. Four working dilutions of the new "Diolaid" disinfectant were experimentally studied according to two 3 (1+2) active components in percentages: 0.04 % (or according to chlorine dioxide 100 mg/dm ); 0.06 % 3 3 (or according to chlorine dioxide 150 mg/dm ); 0.1 % (or chlorine dioxide 250 mg/dm ) and 0.16 % (or 3 chlorine dioxide 400 mg/dm ). The evaluation of the effects of the working solutions of the new disinfectant "Diolaid" was carried out in three repetitions with exposures of 30 and 60 minutes. At the end of the exposure period, to immediately stop the action of the disinfectant, a double volume of sterile physiological solution was added to each of the dilutions, which was then washed three times from the disinfectant residues and sedimentation of the tested test bacteria by centrifugation at 4,000 rpm for 10 min. Test cultures of Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442, washed from the disinfectant, were sown to determine the effects of the bactericidal effect of the test agent - on Petri dishes with tryptone-soy agar, to detect the bacteriostatic effect - on test tubes with tryptone-soy broth and cultivated in thermostats at a temperature of 37±1.0 0C for 24-48 and 48-72 hours, respectively. Based on the analysis of research results, we found that the lowest effective bactericidal concentration 3 of the new disinfectant "Diolide" is 0.06 % (150 mg/dm based on chlorine dioxide), which ensures complete neutralization of test cultures of Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 and not shows a bacteriostatic effect after 30 minutes or longer of contact.