MODES OF COOLING AND DEPRESERVATION OF SEMEN OF ANIMAL (The review)
DOI:
https://doi.org/10.31073/onehealthjournal2024-I-04Keywords:
sperm, cryopreservation, depreservation, media, animals, bulls, stallionsAbstract
An overview of domestic and foreign literary sources is presented, as well as data of published own researches regarding the modes of cryopreservation and depreservation of animal sperm. Ostashko F.I. was the first to clearly prove that the complete disappearance of the liquid phase in the germ cells of wild boars during deep freezing occurs at a temperature of minus 51 oC. Belous A. M. and co-authors showed the dependence of the degree of damage to lysosomes, mitochondria and plasma membranes of sperm depending on the cooling rate. The speed is at the level of 80 oC/min. damages cellular structures to a greater degree than 18 oC/min. In our studies on stallion semen, the dependence of physiological indicators and the degree of damage to cell membranes on cooling modes was proven. Three modes of cryopreservation were used in the experiments: fast - from 0 to minus 10 oC at a rate of 3 oC/min; from minus 10 to minus 80 oC at a rate of 25 oC/min; accelerated (or average) - from 0 to minus 10 °C at a rate of 2.5 °C/min; from minus 10 to minus 80 oC at a rate of 15 oC/min; slow - from 0 to minus 10 oC at a rate of 1 oC/min; from minus 10 to minus 80 oC at a rate of 2.5 oC/min. It has been found that the best results are observed with the rapid cooling method. We also created a method of freezing stallion sperm in 1 ml and 5 ml syringe tubes. For its implementation, a specially developed thermoblock "Cryotrope" is used. In most cases, in practice, a temperature close to the animal's body temperature of 35-40 oC is used for sperm donation. However, the original method of depreservation of bull semen was developed by Bugrov O. D. and Ostashko F. I., which consisted in thawing sperm in coated granules at a temperature of 100 oC. A series of experiments proved that the best physiological indicators of depreserved sperm are observed after keeping the coated granule in boiling water for 6-7 seconds, followed by stabilization in air. At the same time, the average sperm motility was obtained at the level of 46-47 % and the survival time was 9.0−9.2 hours, which significantly exceeded the control. As a result of the long-term work of scientists, methods of cryopreservation of animal sperm have been developed, which allow you to store it for an unlimited time and obtain full-fledged offspring after depreservation.
References
Bezuhlyy M. D., Ostashko F. I. Medvedovskyy O. V. (2001). Instruktsiya zi shtuchnoho osimeninnya velykoyi rohatoyi khudoby (rehionalne vydavnytstvo), Kharkiv, 2001; 32 [in Ukrainian].
Belikov A. A. (1992). Metodika glubokogo zamorazhivaniya i dlitel'nogo khraneniya spermy khryakov-proizvoditeley. Novoye v metodakh zootekhnicheskikh issledovaniy. Institut zhivotnovodstva Ukrainskoy Akademii Agrarnykh Nauk. Kharkіv, Ch.1, 1992; 197−199. [in Ukrainian].
Belous, A.M., Gordienko, E.A., Rozanov, L.F. (1987). Membrane biochemistry. Freezing and cryoprotection. Moscow: Visshaya Shkola, 1987, р.80 [in Russian].
Bugrov, A. D., (1969). Zaplidnennist koriv ta telyts vid osimeninnya zamorozhenoyu spermoyu. Molochno-m’yasne skotarstvo: mizhvidomchyy tematychnyy naukovyy zbirnyk, Kyiv, Urozhay, 1969; 17:80−86. [in Ukrainian].
Gardon, J. C., Castrillo, F., Escribano, B. M., Castejon, F. J. (2008). Equine semen transport with different exstenders and its capability for cryopreservation. Reproduction in domestic Animals; 43(3); July 2008; 99 Budapest. [in Hungary].
Kurbatov, A. D., Platov, Ye. M., Korban, N. V. (1988). Kriokonservacija spermy sel'skohozjajstvennyh zhivotnyh, Leningrad : Agropromizdat, 1988; 256. [in Russian].
Milovanov, V. K. (1947). Svojstvo zhivchikov mlekopitajushhih sohranjat' biologicheskuju polnocennost' posle bystrogo zamorazhivanija : diplom na otkrytiye s prioritetom; 103:1947 [in Russian].
Ostashko, F. I. (1978). Glubokoe zamorazhivanie i dlitel'noe hranenie spermy proizvoditelej Kiyev : Urozhay, 1978, р. 256 [in Ukrainian].
Ostashko, F. I., Bugrov, A. D., (1980). Zashchitnyye svoystva krioprotektorov pri razmorazhivanii spermy, Moscow, Zhivotnovodstvo; 1980; 20−22. [in Russian].
Ostashko, F. I., Bugrov, A. D., (1980). Rozmorozhennya spermy buhayiv- plidnykiv u kyplyachiy vodі, Kyiv, Tvarynnytstvo Ukrayiny No 12, 1980, р. 43−44. [in Ukrainian].
Ostashko, F. I., Bugrov, A. D., (1981). Dekonservatsiya spermy buhayiv, Kyiv, Tvarynnytstvo Ukrayiny No 14, 1981, р. 42−44. [in Ukrainian].
Ostashko, F. I., Bugrov, A. D., Kantsedal V. I. (1982). Metodicheskiye rekomendatsii po organizatsii vosproizvodstva na molochnykh fermakh. Kharkіv, 1982, 32 р. [in Ukrainian].
Parkinson, T. J., Whitfield, C. (1987). Optimisation of freezing conditions for bovine spermatozoa. Theriogenology; 27:781−797.
Pavlenko, B.M., Solovyov, S.T. (2005). Kriokonvektor dlya zamorozhuvannya spermodozy. Ahrarna nauka vyrobnytstvu.– Naukovo-informatsiynyy byuleten zavershenykh naukovykh rozrobok UAAN.; 2(32):26. [in Ukrainian].
Rudenko, E. V., Ostashko, F. I., Sushko, O. B. (2011). Natsionalna tekhnolohiya kriokonservatsiyi ta vykorystannya spermy pleminnykh plidnykiv u systemi krupnomasshtabnoyi selektsiyi. (Kharkiv. Tekhnolohiya aseptychnoho oderzhannya, kriokonservatsiyi spermy buhayiv v oblytsovanykhhranulakh ta shtuchnoho osimeninnya samyts) Ministerstvo ahrarnoyi polityky. Instytut tvarynnytstva Natsionalnoyi Akademiyi Ahrarnykh Nauk, Kharkіv, 2011; 98. [in Ukrainian].
Ricker, J. V., Linfor, J. J., Delfino, W. J., Kysar, P., Scholtz, E. L, Tabli, F., Crowe, J. H., Ball, B. A. and Meyers, S. A. (2006). Equine Sperm Membrane Phase Behavior: The Effect of Lipid-Based Cryoprotectants. Biology of reproduction; 74:359−365.
Sharan, M. M., Yaremchyk, I. M., Nalovylo, S. (2011). Efektyvnist krioprotektoriv roslynnoho i tvarynnoho pokhodzhennya pry zamorozhuvanni spermy buhayiv plidnykiv. Naukovyy visnyk Lvivskoho natsionalnoho universytetu veterynarnoyi medytsyny ta biotekhnolohiy imeni S.Z. Gzhytskoho; 13(50):352−356. [in Ukrainian].
Smirnov, I. V. (1947). To the theory of deep freezing of sperm. Zhivotnovodstvo; 11:65−70. [in Russian].
Smirnov, I. V. (1949). Sohranenie semeni selskohozyaystvennyih zhivotnyih posredstvom glubokogo ohlazhdeniya. Sovetskaya zootehniya; 4:93–96. (in Russian).
Smith, A.U. Polge, C. (1950). Survival of Spermatozoa at Low Temperatures. Nature; 166(4225):668-669, DOI: 10.1038/166668aO
Smith, Audrey U. (1958). Life at Low Temperatures. Nature, 182(4640):911− 913. DOI:10.1038/182911аО
Sokolovskaya, I. I. (1947). Mozhet li zamorozhennaya sperma oplodotvoryat' i davat' normal'noe potomstvo? Doklady VASKhNIL; 6:74 – 75. (in Russian).
Sushko, O. B., Smoronh, Z., Bokhenek, M., Kompaniyets, М., Mishchenko, A. H. (2009). Efektyvnist zastosuvannya bufernykh seredovyshch, krioprotektoriv i rezhymiv zamorozhuvannya spermy zherebtsiv, Kyiv, Visnyk ahrarnoyi nauky, 9:35−39. [in Ukrainian].
Sushko, O. B. (2007). Metod kriokonservatsiyi spermy zherebtsiv z vykorystannyam kompaktyzovanykh shpryts-tyubiv, Kyiv, Visnyk ahrarnoyi nauky, 43−45. [in Ukrainian].
Sushko, O. B., Novikov, O. O., Tkachov, O. V., Soklakova, O. V., Savelyeva, M. S. (2009). Kharkivskyy ekspedytsiyno-statsionaryy metod otrymannya, kriokonservatsiyi spermy ta shtuchnoho osimeninnya koney. Informatsiynyy lystok Instytutu tvarynnytstva Natsionalnoyi Akademiyi Ahrarnykh Nauk (biotsentr, lyutyy), 2009. [in Ukrainian].
Sushko, O. B. (2015). Patentnozakhyshchenyy metod kriokonservuvannya spermy zherebtsiv u shpryts-tyubakh (Kharkivska ekspedytsiyno − statsionarna tekhnolohiya kriokonservuannya spermy ta shtuchnoho osimeninnya koney) Natsionalna akademiya ahrarnykh nauk Ukrayiny, Instytut tvarynnytstva. Metodychni rekomendatsiyi, 2015, р. 15 [in Ukrainian].
Varner, D.D., Blanchard, T.L., Love, C.L., Garcia, M.C., Kenney, R.M. (1988). Effects of cooling rate and storage temperature on equine spermatozoal motility parameters. Animal Reproduction Sciense; 29(5):1043−1051.
Vishwanath, R., Shannon, P. (2000). Storage of bovine semen in liquid and Frozen state. Animal. Reprod. Sci.; 62:25−53.
Zhegunov, G. F., Nardi, O. A., Stegniy, B. Т. (2019). Osnovy kriobiologii i kriomeditsiny: uchebnik dlya studentov – biologov i medikov, 2019, Kharkov.: FLP Brovin A. V. [in Ukrainian].
Yuschenko, N. P., Semakov, V. G., Levin, K. A. (1963). Zamorazhivanie spermyi byikov v granulah bez primeneniya suhogo lda. Molochnoe i myasnoe skotovodstvo; 5:36–37. (in Russian).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 One Health Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
