DETERMINATION OF THE TOXICITY PARAMETERS OF THE BIOCIDAL AGENT "IODOSAN" IN THE LABORATORY ANIMAL MODEL
DOI:
https://doi.org/10.31073/onehealthjournal2024-II-02Keywords:
biocidal agent, concentration, toxicity, laboratory animals, irritating and sensitizing propertiesAbstract
The paper presents the results of laboratory studies of acute toxicity, sensitizing and irritating effects under the conditions of exposure to the biocidal agent "Iodosan" based on iodine and lactic acid in laboratory animal models. Indicators of acute toxicity were determined in clinically healthy white mice. The drug "Yodosan" was administered to animals once intragastrically (by gavage) and by inhalation (treatment of cages). The study of the irritating and sensitizing effects of the drug "Jodosan" in different concentrations was carried out on separate groups of clinically healthy mice by daily dermal application on the back and flanks for 30 days and exposure by immersing the tails of animals in similar concentrations for 30 minutes. Modern humane methods of care and use of laboratory animals were used in the experiment. It was determined that with a single intragastric injection of the biocidal agent "Yodosan", the average lethal dose (LD 50) for white mice was 5515.0 mg/kg body weight. It was proved that the drug "Yodosan" in the form of a 0.3% solution has a temporary irritating and sensitizing effect and has no negative effect on the hematopoiesis in the body of white mice. Thus, based on the results of the study, it was determined that the drug "Iodosan" does not have cumulative and sensitizing properties, does not have irritating and skin-resorptive effects, does not cause changes in hematopoiesis and does not have a suppressive effect on the growth and development of animals, which indicates the environmental safety and harmlessness of the experimental biocidal agent.
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