STUDY OF THE IMMUNOSTIMULATORY EFFECT OF DRONE FEED EXTRACT (ETR) IN THE VACCINATION OF CHICKEN AGAINST NEWCASTLE DISEASE

Abstract

The spread of immunodeficiencies in poultry farming determines the need to find new means of eliminating this severe pathology becomes obvious. To solve this problem, the use of immunostimulants is promising, because they increase the immune status of the body, strengthen natural resistance and reduce the loss of livestock, especially young animals. We developed an immunostimulant, which was made from the larvae of the drone brood - (ETR). This immunostimulant is available, inexpensive and can be produced on the farm. Therefore, we conducted a study on the effectiveness of its use in chickens of different ages when used simultaneously with vaccination against Newcastle disease.
The study was conducted on 28 Leghorn chickens. Four groups were formed according to the principle of analogues. The first and second groups were vaccinated at the age of 15 days with a vaccine from the La Sota strain intranasally, according to the instructions for use, and the chickens of the third and fourth groups were vaccinated at the age of 25 days. To determine the immunostimulating effect of the drug in the first and third groups, together with the vaccination, ETR was drunk in a dose of 0.5 cm3 per individual. The second and fourth groups were controls for their age groups. Blood samples were taken on the 14th day after vaccination.
As a result of the conducted studies, it was established that the received immunostimulant ETR significantly increases the immune response when used simultaneously with vaccination against Newcastle disease. According to the results of serological studies, it was established that in the chickens of the third group, which were vaccinated at the age of 25 days together with the administration of the ETR immunostimulator, the titer of antihemagglutinins accumulated at a titer of 7.3±0.03 log 2, compared with chickens that were administered the immunostimulator at 15 days old, in which the titer of antihemagglutinins accumulated at a titer of 6.9±0.01 log 2.
According to the results of biochemical studies, it was found that the best levels of IgG, Ig M and Ig A were also found in the chickens of the third group and were 6.46±0.024 mg/cm3 , 1.53±0.009 mg/cm3 and 0.70±0.006 mg/cm 3 , respectively. cm3. In chickens of the first group, the level of immunoglobulins was slightly lower, and corresponded to IgG - 6.11±0.013 mg/cm 3 , Ig M - 1.48±0.008 mg/cm3 and Ig A - 0.68±0.006 mg/cm3 . Accordingly, these indicators were significantly lower in the control groups, where only vaccination was carried out.
Thus, the obtained immunostimulant ETR significantly increases the immune response when used simultaneously with vaccination against Newcastle disease. The use of ETR immunostimulator is better for those chickens whose development of immune organs has reached greater functional completion.