Study of the cultural properties of Listeria monocytogenes /Listeria spp under different storage temperature regimes

Authors

DOI:

https://doi.org/10.31073/onehealthjournal2026-I-02

Keywords:

Listeria monocytogenes, isolate, microorganism, strain, quality, CAMP test, hemolytic test, food samples, CFU (colony-forming units)

Abstract

Microbial contamination of food products remains a major public health threat, as consumption of contaminated foods can cause infectious diseases. Among pathogenic bacteria, Listeria monocytogenes occupies a leading position. The significance of listeriosis, caused by L. monocytogenes, is determined by its epizootic, economic, and ecological impact. Pathogenic species of Listeria have become permanent contaminants of food raw materials and food products. Ensuring compliance with microbiological safety requirements remains a critical challenge for food producers, particularly concerning L. monocytogenes contamination. Listeria monocytogenes is a pathogen capable of surviving storage conditions at low temperatures (-25°C) and high temperatures (+45°C). Microorganisms remain viable during prolonged storage in food products at processing plants. Understanding the resistance mechanisms of L. monocytogenes provides essential insight for improving food processing and preservation technologies. The study provides a comprehensive overview of the properties of pathogenic Listeria (at the species level) under different temperature conditions and during long-term storage, which may lead researchers to further implement innovative technologies for the elimination of pathogenic Listeria monocytogenes in food production. Scientists are currently developing new methods for eliminating bacterial contamination in food products and in food industry environments.

References

Bahramia A., Babolia M., Schimmelb K., Jafarid M., Williams L. (2020). Efficiency of novel processing technologies for the control of Listeria monocytogenes in food products. Trends in Food Science & Technology, 62–77. https://doi.org/10.1016/j.tifs.2019.12.009

Shostakovich-Koretska L.R., Budaeva I.V., Nikolaychuk M.A. (2021). Listeriosis: Overview of the problem, presentation of a clinical case of listeriosis sepsis in a 4-month-old child. To Help the Practitioner, 9(4), 39–41. https://doi.org/10.22141/2312-413X.9.4.2021.246482

Sorokina I.V., Miroshnychenko M.S., Pliten O.N., Omelchenko A.A., Kaluzhina A.V., Simacheva A.V., Zvereva I.S. (2016). Clinical and morphological aspects of listeriosis infection in the mother-placenta-fetus system. Morphology. Kharkiv National Medical University, 10(2), 7–9. https://doi.org/10.26641/1997-9665.2016.2.7.-12

Chen J., Regan P., Laksanalamai P., Healey S., Hu Z. (2017). Prevalence and methodologies for detection, characterization, and subtyping of Listeria monocytogenes and L. ivanovii in foods and environmental sources. Food Science and Human Wellness, 6, 97–119. https://doi.org/10.1016/j.ijfoodmicro.2019.108390

Iacumin L., Manzano M., Comi G. (2016). Phage inactivation of Listeria monocytogenes on San Daniele dry-cured ham and elimination of biofilms from equipment and working environments. Microorganisms, 4, 1–12. https://doi.org/10.3390/microorganisms4010004

Kramarenko T., Roasto M., Meremae K., Kuningas M., Poltsama P., Elias T. (2013). Listeria monocytogenes prevalence and serotype diversity in various foods. Food Control, 30, 24–29. https://doi.org/10.1016/j.foodcont.2012.06.047

Buchanan R.L., Gorris L.G., Hayman M.M., Jackson T.C., Whiting R.C. (2017). A review of Listeria monocytogenes: An update on outbreaks, virulence, dose-response, ecology, and risk assessments. Food Control, 1–12. https://doi.org/10.1016/j.foodcont.2016.12.016

Gray J.A., Chandry P.S., Kaur M., Kocharunchitt C., Bowman J.P., Fox E.M. (2018). Novel biocontrol methods for Listeria monocytogenes biofilms in food production facilities. Frontiers in Microbiology, 9, 1–12. https://doi.org/10.3389/fmicb.2018.00605

Feng Y., Yao H., Chen S., Sun X., Yin Y., Jiao X. (2020). Rapid detection of hypervirulent serovar 4h Listeria monocytogenes by multiplex PCR. Frontiers in Microbiology, 11, 1–6. https://doi.org/10.3389/fmicb.2020.01309

Oshchypok I.M. (2020). Technological modes of safe processing of meat raw materials under vacuum. Scientific Papers of the National University of Food Technologies, 124–126. https://doi.org/10.24263/2225-2924-2020-26-5-16

Kanmani P., Rhim J.-W. (2014). Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract. Carbohydrate Polymers, 102, 708–714. https://doi.org/10.1016/j.carbpol.2013.10.099

Kawacka I., Olejnik-Schmidt A., Schmidt M., Sip A. (2020). Effectiveness of phage-based inhibition of Listeria monocytogenes in food products and food processing environments. Microorganisms, 8, 2–19. https://doi.org/10.3390/microorganisms8111764

ISO 11290-2:2017. (2017). Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 2: Enumeration method. https://www.iso.org/ru/standard/60314.html

ISO 7218:2024. (2024). Microbiology of the food chain — General requirements and guidance for microbiological examinations. https://www.iso.org/ru/standard/79508.html

Petruk V., Rudenko Y., Yurchenko A., Kharchenko I., Kharchenko S., Semenikhina O. (2022). Analysis of the results of the pedagogical experiment on the integrated analysis of the average and dispersions. Modern Education and Computer Science, 14, 25–31. https://doi.org/10.5815/ijmecs.2022.06.03

Malaychuk V. P., Astakhov D. S., Litot O. V. (2022). Computer model for studying the

informativeness of the student's test. System Design and Analysis of Aerospace Equipment

Characteristics, 30, 47–54. https://doi.org/10.15421/472205

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Published

2026-01-21

How to Cite

Togachynska, L., Kulykova, V., Halka, I., Ukhovska, T., Ukhovskyi, V., Verkholiuk, M., & Shchur, N. (2026). Study of the cultural properties of Listeria monocytogenes /Listeria spp under different storage temperature regimes. One Health Journal, 4(I), 32–40. https://doi.org/10.31073/onehealthjournal2026-I-02

Issue

Section

Emergent diseases, biosafety and One Health