Experimental evaluation of the bactericidal activity and stability of the biocidal agent "Krezonid"

Authors

DOI:

https://doi.org/10.31073/onehealthjournal2026-III-05

Keywords:

biocidal agents, meta-cresol, bactericidal activity, stability, veterinary disinfection

Abstract

The article presents the results of an experimental assessment of the bactericidal activity and stability of the biocidal agent "Krezonid," whose main active ingredient is meta-cresol (3-methylphenol). The study was conducted to substantiate the effectiveness of the biocidal agent and confirm the preservation of its antimicrobial properties during long-term storage in accordance with the requirements. Bactericidal activity was evaluated using a quantitative suspension method with determination of the number of viable microorganisms (CFU) after exposure to the preparation. Streptococcus faecalis and Salmonella enteritidis strains were used as test cultures. The preparation was studied at concentrations of 0.1, 0.5, and 1.0% with exposure times of 10–30 minutes. The stability of the biocidal agent was studied under real storage conditions for 30 months by monitoring organoleptic indicators, pH values, and the preservation of bactericidal activity. It was found that the biocidal agent "Krezonid" exhibits pronounced bactericidal activity against both test microorganism cultures. Complete inactivation of Streptococcus faecalis and Salmonella enteritidis was achieved at a concentration of 0.5% and exposure of 20 minutes. During storage, the drug "Krezonid" retained its physicochemical homogeneity, stable pH value, and complete bactericidal activity throughout the observation period. The results obtained indicate the high efficacy and stability of the biocidal agent Krezonid, which justifies its use in veterinary practice and the possibility of establishing a shelf life of at least 24 months under regulated storage conditions.

References

Addie D. D. (2018). Phenols and related compounds as antiseptics and disinfectants for use with animals. MSD Veterinary Manual. Retrieved from https://www.msdvetmanual.com/pharmacology/antiseptics-and-disinfectants/phenols-and-related-compounds-as-antiseptics-and-disinfectants-for-use-with-animals?ruleredirectid=445

Britsun V. M., Simurova N. V., Popova I. V., Simurov O. V. (2021). Modern chemical disinfectants and antiseptics. Part II. Journal of Organic and Pharmaceutical Chemistry; 19(4):20–32. doi.:10.24959/ophcj.21.231998

CFSPH (2008–2023). Characteristics of selected disinfectants [PDF]. Center for Food Security and Public Health, Iowa State University. Retrieved from https://www.cfsph.iastate.edu/Disinfection/Assets/characteristics-of-selected-disinfectants.pdf

Boyce J. M. (2023). Quaternary ammonium disinfectants and antiseptics: tolerance, resistance and potential impact on antibiotic resistance. Antimicrobial Resistance & Infection Control; 12:Article 32. doi.:10.1186/s13756-023-01241-z

DIN EN 1656:2010-03 «Khimichni dezinfikuiuchi ta antyseptychni zasoby – kilkisnyi suspensiinii test dlia vyznachennia bakteritsydnoi aktyvnosti khimichnykh i antyseptychnykh zasobiv, yaki zastosovuiutsia v haluzi veterynarii – Metod vyznachennia ta vymohy (faza 2, krok 1)» [Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area – Test method and requirements (phase 2, step 1)]

EN 1040:2004 «Zasoby khimichni dezinfikuiuchi ta antyseptychni. Osnovna bakteritsydna aktyvnist. Chastyna 1. Metod vyprobovuvannia ta vymohy (stadiia 1)» [Chemical disinfectants and antiseptics. Basic bactericidal activity. Part 1. Test method and requirements (phase 1)], metodyk YeS, nyni diiuchoho standarta

Harkavenko T. O., Kovalenko V. L., Horbatiuk O. I., Pinchuk N. H., Kozytska T. H., Harkavenko V. M., Ordynska D. O. (2020). Metodychni rekomendatsii z vyznachennia bakteritsydnoi aktyvnosti ta kontroliu vidsutnosti bakteriostatichnoho efektu dezinfikuiuchykh zasobiv [Methodical recommendations for determining the bactericidal activity and controlling the absence of bacteriostatic effect of disinfectants]. Kyiv: DNDILDZVSE, 2020; 43 p. (in Ukrainian)

Gregirchak N., Lupyna T., Mordych T. (2013). Efektyvnist diyi kombinovanykh dezyinfektantiv [PDF]. Ukrainian Food Journal. Retrieved from https://dspace.nuft.edu.ua/handle/123456789/21357

Krapez P., Lunder M., Oder M., Fink R. (2024). Evaluation of the In Vitro Disinfection Potential of the Phytochemicals Linalool and Citronellal Against Biofilms Formed by Escherichia coli and Staphylococcus aureus. Processes; 12(12):2743. doi.:10.3390/pr12122743

Kovalenko V. L., Nedosiekov V. V. (2011). Metodychni pidkhody shchodo kontroliu dezinfikuiuchykh zasobiv dlia veterynarnoi medytsyny [Methodological approaches to the control of disinfectants for veterinary medicine]. Monohrafiia. Kyiv. 224 p. (in Ukrainian)

Lineback C., Nkemngong C., Wu S., Li X., Teska P., Oliver H. (2018). Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds. Antimicrobial Resistance & Infection Control; 7:154. doi.:10.1186/s13756-018-0447-5

Maillard J.-Y., Pascoe M. (2024). Disinfectants and antiseptics: mechanisms of action and resistance. Nature Reviews Microbiology; 22:4–17. doi.:10.1038/s41579-023-00958-3

Mishra V. K. (2017). Microbial degradation of phenol: A review [PDF]. Research Gate. https://www.researchgate.net/publication/316666855_Microbial_Degradation_of_Phenol_A_Review

Montagna M. T., Triggiano F., Barbuti G., Bartolomeo N., De Giglio O., Diella G., Lopuzzo M., Rutigliano S., Serio G., Caggiano G. (2019). Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria. International Journal of Environmental Research and Public Health; 16(11):1895. doi.:10.3390/ijerph16111895

Oulahal N., Degraeve P. (2022). Phenolic-rich plant extracts with antimicrobial activity: An alternative to food preservatives and biocides? Frontiers in Microbiology; 13:753518. doi:10.3389/fmicb.2021.753518

Palii G. K., Pavliuk S. V., Shevchenko I. P. (2018). Obgruntuvannya zastosuvannya antystepychnykh preparativ u systemi profilaktychnykh i likuvalnykh zakhodiv [PDF]. Bukovynskyy medychnyy visnyk; 22(4):88–98. Retrieved from https://e-bmv.bsmu.edu.ua/article/view/2413-0737.XXII.4.88.2018.98

Ponomarenko G. V., Kovalenko V. L., Kukhtyn M. D., Paliy A. P., Bodnar O. O., Rebenko H. I., Kozytska T. G., Makarevich T. V., Ponomarenko O. V., Palii A. P. (2020). Evaluation of acute toxicity of the «Orgasept» disinfectant. Ukrainian Journal of Ecology; 10(4):273–278. doi.:10.15421/2020_1982

Russell A. D. (2002). Mechanisms of antimicrobial action of antiseptics and disinfectants. Journal of Antimicrobial Chemotherapy; 49(4):597–599. doi.:10.1093/jac/49.4.597

Rutala W. A., Weber D. J. (2019). Disinfection, sterilization, and antisepsis: An overview. American Journal of Infection Control; 47:3–9. doi.:10.1016/j.ajic.2019.01.018

Tako M., Kerekes E. B., Zambrano C., Kotogan A., Papp T., Krisch J., Vagvolgyi C. (2020). Plant phenolics and phenolic-enriched extracts as antimicrobial agents. Antioxidants; 9(2):165. doi.:10.3390/antiox9020165

Tarka P., Nitsch-Osuch A. (2021). Evaluating the virucidal activity of disinfectants according to European Union standards. Viruses; 13(4):534. doi.:10.3390/v13040534

Wales A., Gosling R. J., Bare H. L., Davies R. H. (2020). Disinfectant testing for veterinary and agricultural applications. Zoonoses and Public Health; 67(5):435–447. doi.:10.1111/zph.12830

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Published

2026-05-12

How to Cite

Kovalenko, V., Romanko, M., Ihnatieva, T., Liniichuk, N., Popov, D., Miahka, K., Stupak, O., & Ponomaryova, S. (2026). Experimental evaluation of the bactericidal activity and stability of the biocidal agent "Krezonid". One Health Journal, 4(III), 40–46. https://doi.org/10.31073/onehealthjournal2026-III-05

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Section

Environmental well-being and safety